OGM detection on seed ( corn...)

The Flashkits ® strips detect the presence of transgenic proteins to levels of concentration specific plant GMOs. oooo
SIMPLIFIED PROTOCOL - Manual provided with the full kit.
Contents of Kit X ® Flashkits strips in 1 plastic tube with desiccant. X grams of extraction buffer SEB4 (5.7 g / L).
Not included in the Kit (necessary to use the kit) X litres of distilled water and disposable plastic cups X
Procedure: use a minimum of 100 grains per sample to test.
1. Take a subsample of 20 to 25 grams of seeds.
2. Grind / grind the finest possible this sample grinder with adequate high speed.
3. Put the entire sample and crushed in a disposable plastic cup.
4. Add 40 ml and 60 ml of extraction buffer in the disposable plastic cup.
5. Shake vigorously for 30 seconds, to obtain a homogeneous mixture of seeds and broyat buffer.
6. Allow the solution about 2 minutes (to avoid having too many plant debris suspended).
7. Using a disposable pipette collect 0.5 mL of liquid surface (clear liquid) and the drop in a microvial.
8. Place the strip into the tube (down arrows).
9. Wait 5 to 10 minutes and read the result.
10. For simple testing kits only one type of GMOs
If one line appears, the witness line (top) on the strip, the test is negative.
If 2 lines appear, the witness line (top) and the test line (bottom) the test is positive.
11. For testing kits combined two types GMOs (a witness line and two lines test) If one line appears, the witness line (top) on the strip, the test is negative. If the line of control (above) appears and if one or two lines test (bottom) appear then the test is positive for one or two GMOs. The Flashkits permit if a single test line is colored red to know the GMO concerned.
IMPORTANT: If no line is, the test did not work. Repeat the test with a new strip. If the test or lines (bottom) coloured (s) in green, gray or brown is the test is negative or invalid. These colors are caused by an accumulation of plant debris and dust that clog the membrane and prevents a normal migration to the lines and test witness.
ATTENTION: deep cleaning tools used between two checks. The sampling and the number of samples remain the basis of a representative. BIOFORDS could not be held responsible for the consequences of sampling poorly drawn or an insufficient number of samples, and therefore not statistically representative of a lot of seeds or seeds. For a representative sample, according to the settlement and the batch control, consult the tables statistics.
ADDITIONAL INSTRUCTIONS: take care store Flashkits ® strips unused in their original packaging, with the desiccant.
DO NOT FOLD THE STRIPS.
Keep cool to 4 / 6 ° Celsius