Stone Fruits

Plum Pox Virus

PPV is a potyvirus that affects stone fruit trees causing a disease known as Plum Pox or Sharka. This disease can drastically decrease the quality and the productivity of stone fruit trees and is transmitted from one plant to another by the feeding of several species of aphids.

This new kit, based on polyclonal antibodies, can detect all PPV isolates: PPV-C, PPV-M, PPV-D, PPV-EA, PPV-W as well as the recombinant strain, PPV-Rec.

This test has been developed in collaboration with Dr. D’Ann Rochon from the Agriculture & Agri-Food in Canada. The test has been then evaluated using the INRA - Bordeaux collection of PPV isolates and at AAFC and USDA ARS - APHIS.

Agdia Biofords participates in the U. S National Plum Pox Virus Survey program, under the direction and guidance of USDA-APHIS. Agdia PPV ELISA kits have been used in the U. S. National PPV Survey for the last 3 years.

ELISA kit formats is a double antibody sandwich (DAS) ELISA, offered as a Reagent Set. It is available in 4 formats: 96, 500, 1000 and 5000 estwells. Incubation times are flexible: 2 hour at RT or overnight, allowing to complete the test in 8 hours. Post coat buffer allows you to coat and store plates several weeks prior to testing. Each Reagent set includes stable and non-infectious positive controls on a strip. Multiple strip format allows user to prepare only the amount of positive control needed, fresh, each time.